116 kDa U5 small nuclear ribonucleoprotein component, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Bovine EFTUD2. No significant cross-reactivity or interference between Bovine EFTUD2 and analogues was observed.

Store at 2-8 degree C.

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Small volumes of EFTUD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281191 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 116 kDa U5 small nuclear ribonucleoprotein component (EFTUD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EFTUD2. The ELISA analytical biochemical technique of the MBS281191 kit is based on EFTUD2 antibody-EFTUD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EFTUD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EFTUD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

EFTUD2 is structurally closely related to the eukaryotic translation elongation factor EF2, sharing 27 to 50% sequence identity depending on the region examined. this protein bound GTP specifically. Immunofluorescence microscopy revealed that U5-116kD is located in the nucleus of HeLa cells and colocalizes with snRNP-containing nuclear speckles.
Antibodies against U5-116kD inhibited pre-mRNA splicing in a HeLa nuclear extract. These authors also identified homologs in S. cerevisiae, C. elegans, and mouse, designating them Snu114p, Caeel-116H, and U5-116kD, respectively. Human U5-116kD shares 32%, 74%, and 99% identity with Snu114p, Caeel-116H, and mouse U5-116kD, respectively

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EFTUD2 in samples. An antibody specific for EFTUD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFTUD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFTUD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFTUD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

109,386 Da

elongation factor Tu GTP binding domain containing 2

116 kDa U5 small nuclear ribonucleoprotein component

116 kDa U5 small nuclear ribonucleoprotein component

SNRP116; U5-116 kDa  [Similar Products]

Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP ().

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