Sodium/glucose cotransporter 1, ELISA Kit

Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1; Slc5a1; Sglt1

For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural rat Na(+)/glucose cotransporter 1. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Slc5a1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS289471 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/glucose cotransporter 1 (Slc5a1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Slc5a1. The ELISA analytical biochemical technique of the MBS289471 kit is based on Slc5a1 antibody-Slc5a1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Slc5a1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Slc5a1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat sodium/glucose cotransporter 1, Na(+)/glucose cotransporter 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na(+)/glucose cotransporter 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Na(+)/glucose cotransporter 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Na(+)/glucose cotransporter 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Na(+)/glucose cotransporter 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

73,066 Da

solute carrier family 5 (sodium/glucose cotransporter), member 1

sodium/glucose cotransporter 1

Sodium/glucose cotransporter 1

Sglt1; Na(+)/glucose cotransporter 1  [Similar Products]

SC5A1_RAT

catalyzes sodium dependent glucose transport; plays a role in sugar absorption in the small intestine and reabsorption of sugars in the kidney [RGD, Feb 2006]

SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Cellular Component: apical plasma membrane; brush border; brush border membrane; integral to membrane; intercellular junction

Molecular Function: glucose transmembrane transporter activity; glucose:sodium symporter activity

Biological Process: D-glucose absorption; glucose transport; intestinal absorption; metanephros development; sodium ion transport

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