ICE protease-activating factor (IRAP), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of IRAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2600488 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ICE protease-activating factor (IRAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IRAP. The ELISA analytical biochemical technique of the MBS2600488 kit is based on IRAP antibody-IRAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

10,646 Da

NLR family, CARD domain containing 4

NLR family CARD domain-containing protein 4; NOD-like receptor C4; ICE-protease activating factor; ice protease-activating factor; CARD, LRR, and NACHT-containing protein; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4

NLR family CARD domain-containing protein 4

CARD12; CLAN; CLAN1; IPAF; UNQ6189/PRO20215; Clan protein; Ipaf  [Similar Products]

NLRC4_HUMAN

In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM, Mar 2008]

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: spindle microtubule; cytoplasm; intracellular; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; magnesium ion binding; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ATP binding

Biological Process: caspase activation; detection of bacterium; positive regulation of apoptosis; protein ubiquitination; regulation of signal transduction; activation of NF-kappaB transcription factor; defense response to bacterium; regulation of neuron apoptosis; innate immune response; interleukin-1 beta secretion; inflammatory response; activation of innate immune response; protein homooligomerization; negative regulation of apoptosis

Disease: Autoinflammation With Infantile Enterocolitis; Familial Cold Autoinflammatory Syndrome 4

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