Full Product Name
PHF6 siRNA (Human)
Product Synonym Names
KIAA1823; PHD finger protein 6; PHD-like zinc finger protein
Product Gene Name
PHF6 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8IWS0
Specificity
PHF6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PHF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PHF6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PHF6 sirna
siRNA to inhibit PHF6 expression using RNA interference
Applications Tested/Suitable for PHF6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PHF6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001015877.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001015877.1
[Other Products]
UniProt Primary Accession #
Q8IWS0
[Other Products]
UniProt Secondary Accession #
Q5JRC7; Q5JRC8; Q96JK3; Q9BRU0; A8K230; B4E0G4; D3DTG3; E9PC97[Other Products]
UniProt Related Accession #
Q8IWS0[Other Products]
Molecular Weight
37,628 Da
NCBI Official Full Name
PHD finger protein 6 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 6
NCBI Official Symbol
PHF6 [Similar Products]
NCBI Official Synonym Symbols
BFLS; BORJ; CENP-31
[Similar Products]
NCBI Protein Information
PHD finger protein 6
UniProt Protein Name
PHD finger protein 6
UniProt Synonym Protein Names
PHD-like zinc finger protein
Protein Family
PHD finger protein
UniProt Gene Name
PHF6 [Similar Products]
UniProt Synonym Gene Names
CENP-31; KIAA1823 [Similar Products]
UniProt Entry Name
PHF6_HUMAN
NCBI Summary for PHF6
This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate splicing results in multiple transcript variants, encoding different isoforms. [provided by RefSeq, Jun 2010]
UniProt Comments for PHF6
PHF6: May play a role in transcriptional regulation. Defects in PHF6 are the cause of Boerjeson-Forssman- Lehmann syndrome (BFLS); also known as Boerjeson- Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function; Nucleolus
Chromosomal Location of Human Ortholog: Xq26.3
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: tubulin binding; ribonucleoprotein binding; protein binding; enzyme binding; DNA binding; histone binding; zinc ion binding; histone deacetylase binding; phosphoprotein binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Disease: Borjeson-forssman-lehmann Syndrome
Research Articles on PHF6
1. Female phenotypes of Borjeson-Forssman-Lehmann syndrome patients with PHF6 mutations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
