Full Product Name
HEXB Antibody
Product Synonym Names
beta-hexosaminidase beta; beta-N-acetylhexosaminidase; cervical cancer proto-oncogene 7; HCC-7
Product Gene Name
anti-HEXB antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07686
Specificity
HEXB Antibody detects endogenous levels of total HEXB protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human HEXB.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-HEXB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HEXB antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-HEXB antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Immunohistochemistry (IHC) of anti-HEXB antibody
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using HEXB Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot (WB) of anti-HEXB antibody
Western blot analysis of lysates from Jurkat cells, using HEXB Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for HEXB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000512.1
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NCBI GenBank Nucleotide #
NM_000521.3
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UniProt Primary Accession #
P07686
[Other Products]
UniProt Related Accession #
P07686[Other Products]
NCBI Official Full Name
beta-hexosaminidase subunit beta isoform 1 preproprotein
NCBI Official Synonym Full Names
hexosaminidase subunit beta
NCBI Official Symbol
HEXB [Similar Products]
NCBI Official Synonym Symbols
ENC-1AS; HEL-248; HEL-S-111
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NCBI Protein Information
beta-hexosaminidase subunit beta
UniProt Protein Name
Beta-hexosaminidase subunit beta
UniProt Synonym Protein Names
Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B
Protein Family
Beta-hexosaminidase
UniProt Gene Name
HEXB [Similar Products]
UniProt Synonym Gene Names
Hexosaminidase subunit B; HCC-7 [Similar Products]
NCBI Summary for HEXB
Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for HEXB
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Research Articles on HEXB
1. a modified human hexosaminidase subunit beta (HexB), which we have termed mod2B, composed of homodimeric beta subunits that contain amino acid sequences from the alpha subunit that confer GM2 ganglioside-degrading activity and protease resistance.
Precautions
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Disclaimer
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