Product Name
AKR1D1, Polyclonal Antibody
Full Product Name
Anti-AKR1D1 Antibody
Product Gene Name
anti-AKR1D1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human. Predicted: Mouse, Rat
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Recombinate human AKR1D1 protein 73-315aa (BC130625)
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-AKR1D1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AKR1D1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AKR1D1 antibody
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user
Western Blot (WB) of anti-AKR1D1 antibody
Immunohistochemistry (IHC) #2 of anti-AKR1D1 antibody
NCBI/Uniprot data below describe general gene information for AKR1D1. It may not necessarily be applicable to this product.
NCBI Accession #
BC130625
[Other Products]
UniProt Secondary Accession #
A1L4P6; A8K060; B4DPN3; B4DPN8[Other Products]
UniProt Related Accession #
P51857[Other Products]
Molecular Weight
32,890 Da
NCBI Official Full Name
Homo sapiens aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase), mRNA
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member D1
NCBI Official Symbol
AKR1D1 [Similar Products]
NCBI Official Synonym Symbols
CBAS2; SRD5B1; 3o5bred
[Similar Products]
NCBI Protein Information
3-oxo-5-beta-steroid 4-dehydrogenase; delta 4-3-ketosteroid-5-beta-reductase; delta(4)-3-ketosteroid 5-beta-reductase; delta(4)-3-oxosteroid 5-beta-reductase; steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
UniProt Protein Name
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Synonym Protein Names
Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase
Protein Family
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Gene Name
AKR1D1 [Similar Products]
UniProt Entry Name
AK1D1_HUMAN
NCBI Summary for AKR1D1
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]
UniProt Comments for AKR1D1
AKR1D1: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. Defects in AKR1D1 are the cause of congenital bile acid synthesis defect type 2 (CBAS2); also known as cholestasis with delta(4)-3-oxosteroid 5-beta-reductase deficiency. Patients with this liver disease show absence or low levels of chenodeoxycholic acid and cholic acid in plasma and urine. Belongs to the aldo/keto reductase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; EC 1.3.1.3
Chromosomal Location of Human Ortholog: 7q32-q33
Cellular Component: cytosol
Molecular Function: delta4-3-oxosteroid 5beta-reductase activity; steroid binding
Biological Process: bile acid biosynthetic process; bile acid metabolic process; bile acid catabolic process; C21-steroid hormone metabolic process; androgen metabolic process; digestion; cholesterol catabolic process
Disease: Bile Acid Synthesis Defect, Congenital, 2
Product References and Citations for anti-AKR1D1 antibody
Steen,N.E., Tesli,M., Kahler,A.K., et al. SRD5A2 is associated with increased cortisol metabolism in schizophrenia spectrum disorders. Prog. Neuropsychopharmacol. Biol. Psychiatry 34 (8), 1500-1506 (2010)
Research Articles on AKR1D1
1. AKR1D1 generates all 5beta-dihydrosteroids in the C19-C27 steroid series.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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