COL10A1, Polyclonal Antibody

Collagen alpha-1(X) chain; COL10A1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This COL10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human COL10A1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-COL10A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes the alpha chain of type X collagen, a
short chain collagen expressed by hypertrophic chondrocytes during
endochondral ossification. Unlike type VIII collagen, the other
short chain collagen, type X collagen is a homotrimer. Mutations in
this gene are associated with Schmid type metaphyseal
chondrodysplasia (SMCD) and Japanese type spondylometaphyseal
dysplasia (SMD).

Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

COL10A1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the COL10A1 antibody detected the COL10A1 protein (arrow).

66158

collagen, type X, alpha 1

collagen alpha-1(X) chain

Collagen alpha-1(X) chain

COAA1_HUMAN

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type

Makitie, O., et al. J. Orthop. Res. 28(11):1497-1501(2010)
Wang, S., et al. Connect. Tissue Res. 51(3):188-196(2010)
Videman, T., et al. Arthritis Rheum. 60(2):470-481(2009)
Higashikawa, A., et al. Arthritis Rheum. 60(1):166-178(2009)
Zimmermann, P., et al. Arthritis Rheum. 58(9):2743-2753(2008)

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