Product Name
PCSK1, siRNA
Full Product Name
PCSK1 siRNA (Human)
Product Synonym Names
NEC1; Neuroendocrine convertase 1; NEC 1; Prohormone convertase 1; Proprotein convertase 1; PC1
Product Gene Name
PCSK1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P29120
Specificity
PCSK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PCSK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PCSK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PCSK1 sirna
siRNA to inhibit PCSK1 expression using RNA interference
Applications Tested/Suitable for PCSK1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PCSK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000430.3
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NCBI GenBank Nucleotide #
NM_000439.4
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UniProt Primary Accession #
P29120
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UniProt Secondary Accession #
P78478; Q92532; B7Z8T7; E9PHA1[Other Products]
UniProt Related Accession #
P29120[Other Products]
Molecular Weight
79,010 Da
NCBI Official Full Name
neuroendocrine convertase 1 isoform 1 preproprotein
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 1
NCBI Official Symbol
PCSK1 [Similar Products]
NCBI Official Synonym Symbols
PC1; PC3; NEC1; SPC3; BMIQ12
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NCBI Protein Information
neuroendocrine convertase 1
UniProt Protein Name
Neuroendocrine convertase 1
UniProt Synonym Protein Names
Prohormone convertase 1; Proprotein convertase 1; PC1
Protein Family
Neuroendocrine convertase
UniProt Gene Name
PCSK1 [Similar Products]
UniProt Synonym Gene Names
NEC1; NEC 1; PC1 [Similar Products]
UniProt Entry Name
NEC1_HUMAN
NCBI Summary for PCSK1
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]
UniProt Comments for PCSK1
PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.
Protein type: Protease; EC 3.4.21.93
Chromosomal Location of Human Ortholog: 5q15-q21
Cellular Component: Golgi apparatus; extracellular space; transport vesicle
Molecular Function: serine-type endopeptidase activity
Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process
Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12
Research Articles on PCSK1
1. Epistases between single nucleotide polymorphisms within proprotein convertase subtilisin/kexin type 1(PCSK1) and dopamine beta-hydroxylase(DBH) genes are significantly associated with susceptibility or resistance to premature ovarian failure
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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