Product Name
PCSK1 / PC1, Monoclonal Antibody
Popular Item
Full Product Name
Mouse Monoclonal [clone 3D2] (IgG3,k) to Human PCSK1 / PC1
Product Synonym Names
Anti-PCSK1 / PC1 Antibody (clone 3D2) IHC-plus; PCSK1; Pc1/3; PC1; Prohormone convertase 3; SPC3; Neuroendocrine convertase 1; PC3; Prohormone convertase 1; BMIQ12; NEC 1; NEC1; Proprotein convertase 1; Human PCSK1; PC1
Product Gene Name
anti-PCSK1 antibody
[Similar Products]
Product Synonym Gene Name
PC1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P29120
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Purified fromascites by Protein A
Concentration
0.48 mg/ml (lot specific)
Family/Subfamily
Protease/Serine S8
Antigen Type
Recombinant Protein
Immunogen
PCSK1 (NP_000430, 652 a.a. ~ 754 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
PCSK1 / PC1 antibody was raised against pCSK1 (NP_000430, 652 a.a. ~ 754 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-PCSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PCSK1 antibody
PCSK1 is a Serine S8 type protease.
Product Categories/Family for anti-PCSK1 antibody
Subfamily:-Serine-S8">Family: Protease
Subfamily: Serine S8
Applications Tested/Suitable for anti-PCSK1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PCSK1 antibody
ELISA, IF (30 ug/ml), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections.
Sandwich ELISA: Recombinant protein.
Western Blot: using cell lysates and the recombinant protein used for the immunogen.
Optimal dilution to be determined by the researcher.
Immunohistochemistry (IHC) of anti-PCSK1 antibody
Anti-PCSK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Immunofluorescence (IF) of anti-PCSK1 antibody
Immunofluorescence of monoclonal antibody to PCSK1 on HeLa cell. [antibody concentration 30 ug/ml]
Western Blot (WB) of anti-PCSK1 antibody
PCSK1 monoclonal antibody clone 3D2. Western blot of PCSK1 expression in NIH/3T3.
Western Blot (WB) of anti-PCSK1 antibody
PCSK1 monoclonal antibody clone 3D2. Western blot of PCSK1 expression in PC-12.
ELISA (EIA) of anti-PCSK1 antibody
Detection limit for recombinant GST tagged PCSK1 is approximately 0.03 ng/ml as a capture antibody.
NCBI/Uniprot data below describe general gene information for PCSK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000430.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000439.4
[Other Products]
UniProt Primary Accession #
P29120
[Other Products]
UniProt Secondary Accession #
P78478; Q92532; B7Z8T7; E9PHA1[Other Products]
UniProt Related Accession #
P29120[Other Products]
Molecular Weight
79,010 Da
NCBI Official Full Name
neuroendocrine convertase 1 isoform 1 preproprotein
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 1
NCBI Official Symbol
PCSK1 [Similar Products]
NCBI Official Synonym Symbols
PC1; PC3; NEC1; SPC3; BMIQ12
[Similar Products]
NCBI Protein Information
neuroendocrine convertase 1; prohormone convertase 1; prohormone convertase 3
UniProt Protein Name
Neuroendocrine convertase 1
UniProt Synonym Protein Names
Prohormone convertase 1; Proprotein convertase 1; PC1
Protein Family
Neuroendocrine convertase
UniProt Gene Name
PCSK1 [Similar Products]
UniProt Synonym Gene Names
NEC1; NEC 1; PC1 [Similar Products]
UniProt Entry Name
NEC1_HUMAN
NCBI Summary for PCSK1
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]
UniProt Comments for PCSK1
PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.
Protein type: EC 3.4.21.93; Protease
Chromosomal Location of Human Ortholog: 5q15-q21
Cellular Component: Golgi apparatus; extracellular space; transport vesicle
Molecular Function: serine-type endopeptidase activity
Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process
Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12
Research Articles on PCSK1
1. Common genetic variants in PCSK1 are associated with coronary artery disease in Chinese patients with type 2 diabetes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
