GABA A Receptor Alpha-1, Monoclonal Antibody

Mouse Anti-Human GABA-A Receptor Alpha-1 Monoclonal IgG2A; GABA-A Receptor Alpha-1 Antibody: Dylight 594; ECA4 antibody; EJM antibody; EJM5 antibody; Gaba receptor alpha 1 polypeptide antibody; GABA(A) receptor antibody; GABA(A) receptor subunit alpha 1 antibody; GABA(A) receptor subunit alpha-1 antibody; GABA(A) receptor; alpha 1 antibody; GABRA 1 antibody; GABRA1 antibody; Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody; Gamma aminobutyric acid A receptor alpha 1 antibody; Gamma aminobutyric acid receptor subunit alpha 1 antibody; Gamma-aminobutyric acid receptor subunit alpha-1 antibody; GBRA1_HUMAN antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2A

S95-35

Mouse

Detects ~55 kDa. No cross-reactivity against GABA-A-R-Alpha 2 or-Alpha3.

Protein G Purified

PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide

1 mg/ml (lot specific)

Store at-20 degree C.

Small volumes of anti-GABA A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).

Neuroscience; Neurotransmitter Receptors; GABA Receptors; GABAA Receptors

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Microarray (AM)

WB: 1:1000
IHC: 1:1000
ICC/IF: 1:100

Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35 at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

gamma-aminobutyric acid type A receptor alpha1 subunit

gamma-aminobutyric acid receptor subunit alpha-1

Gamma-aminobutyric acid receptor subunit alpha-1

GBRA1_HUMAN

This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

GABRA1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4). A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into *****hood. Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5). A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

Protein type: Membrane protein, integral; Channel, ligand-gated; Transporter, ion channel; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; GABA receptor activity; drug binding; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; transport; synaptic transmission, GABAergic; transmembrane transport; gamma-aminobutyric acid signaling pathway

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 13; Epileptic Encephalopathy, Early Infantile, 19

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.

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