GABA alpha1beta3, Cell Line

H-alpha1beta3; Heteropentamer Human GABA alpha1beta3 Stable Cell Line; GABA(A) receptor subunit alpha-1/GABA(A) receptor subunit beta-3

For Research Use Only. Not for use in diagnostic procedures.

3.106 cells/mL in freezing medium
Cells: 2 x 1 mL frozen aliquots

Store in liquid nitrogen (vapor phase) immediatly upon receipt.
Stability: The cells were kept in continuous culture for at least 60 days and showed no decrease of receptor expression level.

Small volumes of GABRA1/GABRB3 cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This stable cell line expresses a recombinant human GABA alpha1beta3. This tetracycline inducible cell line carries a double twin-strep-tag and a Flag tag. There is a TEV cleavage site inserted between the tag and the receptor for tag's removal.
Tetracycline inducible recombinant heteropentamer human gamma-aminobutyric acid receptor subunit alpha-1 and beta-3 stable HEK cell line.

Human gamma-aminobutyric acid receptor subunit alpha-1 and beta-3 stable KEK-TREx cell line was established using Hygromycin at 150pg/mL and Zeocin at IDOpg/mL. This monoclonal cell line were obtained by limit dilution allowing a homogeneous expression, assesed by flow cytometry (FACS).

Cell Lines For Membrane Receptor Research; Ion Channel Stable Cell Lines

51,802 Da

gamma-aminobutyric acid type A receptor alpha1 subunit

gamma-aminobutyric acid receptor subunit alpha-1

Gamma-aminobutyric acid receptor subunit alpha-1

This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector . Functions as ligand-gated chloride channel ().

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