Full Product Name
CISD2 Polyclonal Antibody
Product Synonym Names
ERIS; Miner1; NAF-1; WFS2; ZCD2
Product Gene Name
anti-CISD2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
PKKKQQKDSL INLKIQKENP KVVNEINIED LCLTKAAYCR CWRSKTFPAC DGSHNKHNEL TGDNVGPLIL KKKEV
3D Structure
ModBase 3D Structure for Q8N5K1
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human CISD2
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Endoplasmic reticulum membrane, Mitochondrion outer membrane, Single-pass membrane protein
Positive Samples
293T, A549, K562, Mouse pancreas
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-CISD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CISD2 antibody
The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.
Product Categories/Family for anti-CISD2 antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-CISD2 antibody
Western Blot (WB)
Application Notes for anti-CISD2 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-CISD2 antibody
Western blot analysis of extracts of various cell lines, using CISD2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
NCBI/Uniprot data below describe general gene information for CISD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001008389.1
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NCBI GenBank Nucleotide #
NM_001008388.4
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UniProt Primary Accession #
Q8N5K1
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UniProt Secondary Accession #
Q7Z3D5[Other Products]
UniProt Related Accession #
Q8N5K1[Other Products]
Molecular Weight
Calculated: 15kDa
Observed: 15kDa
NCBI Official Full Name
CDGSH iron-sulfur domain-containing protein 2
NCBI Official Synonym Full Names
CDGSH iron sulfur domain 2
NCBI Official Symbol
CISD2 [Similar Products]
NCBI Official Synonym Symbols
ERIS; WFS2; ZCD2; NAF-1; Miner1
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NCBI Protein Information
CDGSH iron-sulfur domain-containing protein 2
UniProt Protein Name
CDGSH iron-sulfur domain-containing protein 2
UniProt Synonym Protein Names
Endoplasmic reticulum intermembrane small protein; MitoNEET-related 1 protein; Miner1; Nutrient-deprivation autophagy factor-1; NAF-1
Protein Family
CDGSH iron-sulfur domain-containing protein
UniProt Gene Name
CISD2 [Similar Products]
UniProt Synonym Gene Names
CDGSH2; ERIS; ZCD2; Miner1; NAF-1 [Similar Products]
NCBI Summary for CISD2
The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]
UniProt Comments for CISD2
Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.
Research Articles on CISD2
1. A mutation resulted in the loss of functional CISD2 protein in the two Italian Wolfram syndrome type 2 patients.">The c.103 + 1G > A mutation resulted in the loss of functional CISD2 protein in the two Italian Wolfram syndrome type 2 patients.
Precautions
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Disclaimer
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