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HES7, siRNA

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产品名称: HES7, siRNA
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简单介绍

HES7, siRNA


HES7, siRNA  的详细介绍
Product Name

HES7, siRNA

Full Product Name

HES7 siRNA (Mouse)

Product Synonym Names
Transcription factor HES-7; mHes7; Hairy and enhancer of split 7; bHLH factor Hes7
Product Gene Name

HES7 sirna

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for Q8BKT2
Host
Synthetic
Species Reactivity
Mouse
Specificity
HES7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HES7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HES7 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
HES7 sirna
siRNA to inhibit HES7 expression using RNA interference
Applications Tested/Suitable for HES7 sirna
RNA Interference (RNAi)
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NCBI/Uniprot data below describe general gene information for HES7. It may not necessarily be applicable to this product.
NCBI GI #
61098129
NCBI GeneID
84653
NCBI Accession #
NP_149030.2 [Other Products]
NCBI GenBank Nucleotide #
NM_033041.4 [Other Products]
UniProt Primary Accession #
Q8BKT2 [Other Products]
UniProt Secondary Accession #
Q99JA6; A3KPD3[Other Products]
UniProt Related Accession #
Q8BKT2[Other Products]
Molecular Weight
24,890 Da
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NCBI Official Full Name
transcription factor HES-7
NCBI Official Synonym Full Names
hairy and enhancer of split 7 (Drosophila)
NCBI Official Symbol
Hes7  [Similar Products]
NCBI Official Synonym Symbols
bHLHb37
  [Similar Products]
NCBI Protein Information
transcription factor HES-7
UniProt Protein Name
Transcription factor HES-7
UniProt Synonym Protein Names
Hairy and enhancer of split 7; bHLH factor Hes7
Protein Family
Transcription factor
UniProt Gene Name
Hes7  [Similar Products]
UniProt Synonym Gene Names
mHes7  [Similar Products]
UniProt Entry Name
HES7_MOUSE
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UniProt Comments for HES7
HES7: Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation. Defects in HES7 are the cause of spondylocostal dysostosis type 4 (SCDO4). A rare condition of variable severity characterized by vertebral and costal anomalies. The main feature include dwarfism, vertebral fusion, hemivertebrae, posterior rib fusion, reduced rib number, and other rib malformations.

Cellular Component: nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription factor binding

Biological Process: somitogenesis; Notch signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; regulation of somitogenesis; negative regulation of transcription, DNA-dependent; skeletal development
Research Articles on HES7
1. Lengthening the third Hes7 intron by 10 or 20 kb disrupts accurate RNA splicing and inactivates the gene.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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