Werner syndrome, RecQ helicase-like, ELISA Kit

Human Werner syndrome ATP-dependent helicase (WRN) ELISA kit; DKFZp686C2056; RECQ3; RECQL2; RECQL3; Werner syndrome protein; Werner syndrome; RecQ helicase-like

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of WRN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9333349 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Werner syndrome, RecQ helicase-like (WRN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WRN. The ELISA analytical biochemical technique of the MBS9333349 kit is based on WRN antibody-WRN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WRN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WRN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

162,461 Da

Werner syndrome, RecQ helicase-like

Werner syndrome ATP-dependent helicase; exonuclease WRN; recQ protein-like 2; DNA helicase, RecQ-like type 3

Werner syndrome ATP-dependent helicase

RECQ3; RECQL2; RecQ3  [Similar Products]

WRN_HUMAN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: DNA repair, damage; DNA-binding; Cell cycle regulation; Helicase; EC 3.6.4.12; Nucleolus

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: MutLalpha complex; nucleoplasm; centrosome; nucleolus

Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; helicase activity; four-way junction helicase activity; ATP-dependent DNA helicase activity; Y-form DNA binding; protein binding; DNA helicase activity; ATP-dependent 3'-5' DNA helicase activity; DNA binding; manganese ion binding; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; ATP binding

Biological Process: nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; cell aging; multicellular organismal aging; replicative cell aging; cellular response to starvation; response to UV-C; DNA duplex unwinding; DNA recombination; replication fork processing; regulation of apoptosis; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; response to oxidative stress; regulation of growth rate; DNA replication; DNA metabolic process; response to DNA damage stimulus; telomere maintenance; aging

Disease: Werner Syndrome

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