Full Product Name
WRN Rabbit Polyclonal
Product Gene Name
anti-WRN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Immunogen
Werner syndrome, RecQ helicase-like
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-WRN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-WRN antibody
ELISA (EIA), Western Blot (WB)
NCBI/Uniprot data below describe general gene information for WRN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000544.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000553.4
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UniProt Secondary Accession #
A1KYY9[Other Products]
UniProt Related Accession #
Q14191[Other Products]
Molecular Weight
162,461 Da
NCBI Official Full Name
Werner syndrome ATP-dependent helicase
NCBI Official Synonym Full Names
Werner syndrome RecQ like helicase
NCBI Official Symbol
WRN [Similar Products]
NCBI Official Synonym Symbols
RECQ3; RECQL2; RECQL3
[Similar Products]
NCBI Protein Information
Werner syndrome ATP-dependent helicase
UniProt Protein Name
Werner syndrome ATP-dependent helicase
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 3; RecQ3; Exonuclease WRN (EC:3.1.-.-); RecQ protein-like 2
Protein Family
Werner syndrome ATP-dependent helicase
UniProt Gene Name
WRN [Similar Products]
UniProt Synonym Gene Names
RECQ3; RECQL2; RecQ3 [Similar Products]
UniProt Entry Name
WRN_HUMAN
NCBI Summary for WRN
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]
UniProt Comments for WRN
WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
Protein type: EC 3.6.4.12; DNA repair, damage; Cell cycle regulation; Helicase; Nucleolus; DNA-binding
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: centrosome; cytoplasm; MutLalpha complex; neuron projection; nuclear chromosome, telomeric region; nucleolus; nucleoplasm
Molecular Function: 3'-5' DNA helicase activity; 3'-5' exonuclease activity; ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATPase activity; bubble DNA binding; chromatin binding; DNA binding; DNA helicase activity; exonuclease activity; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; magnesium ion binding; manganese ion binding; protein binding; protein complex binding; protein homodimerization activity; Y-form DNA binding
Biological Process: aging; base-excision repair; brain development; cell aging; cellular response to starvation; DNA duplex unwinding; DNA metabolic process; DNA replication; DNA synthesis during DNA repair; double-strand break repair; multicellular organismal aging; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; protein sumoylation; regulation of apoptosis; regulation of growth rate; replication fork processing; replicative cell aging; response to DNA damage stimulus; response to oxidative stress; response to UV-C; strand displacement; telomere maintenance; telomere maintenance via recombination
Disease: Werner Syndrome
Research Articles on WRN
1. Multiplex genetic testing of 90 cancer susceptibility genes disclosed a novel WRN frameshift mutation (p.N1370Tfs*23) in a mother and her 2 daughters with cancer and in the youngest unaffected daughter.
Precautions
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Disclaimer
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