Product Name
SLC37A4, siRNA
Full Product Name
SLC37A4 siRNA (Human)
Product Synonym Names
G6PT; G6PT1; Glucose-6-phosphate translocase; Glucose-5-phosphate transporter; Solute carrier family 37 member 4; Transformation-related gene 19 protein; TRG-19
Product Gene Name
SLC37A4 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43826
Specificity
SLC37A4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC37A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC37A4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC37A4 sirna
siRNA to inhibit SLC37A4 expression using RNA interference
Applications Tested/Suitable for SLC37A4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC37A4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001157749.1
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NCBI GenBank Nucleotide #
NM_001164277.1
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UniProt Primary Accession #
O43826
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UniProt Secondary Accession #
O96016; Q5J7V4; Q9UI19; Q9UNS4[Other Products]
UniProt Related Accession #
O43826[Other Products]
Molecular Weight
48,840 Da
NCBI Official Full Name
glucose-6-phosphate translocase isoform 1
NCBI Official Synonym Full Names
solute carrier family 37 (glucose-6-phosphate transporter), member 4
NCBI Official Symbol
SLC37A4 [Similar Products]
NCBI Official Synonym Symbols
G6PT1; G6PT2; G6PT3; GSD1b; GSD1c; GSD1d; TRG19; TRG-19; PRO0685
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NCBI Protein Information
glucose-6-phosphate translocase
UniProt Protein Name
Glucose-6-phosphate translocase
UniProt Synonym Protein Names
Glucose-5-phosphate transporter; Solute carrier family 37 member 4; Transformation-related gene 19 protein; TRG-19
Protein Family
Glucose-6-phosphate exchanger
UniProt Gene Name
SLC37A4 [Similar Products]
UniProt Synonym Gene Names
G6PT; G6PT1; TRG-19 [Similar Products]
UniProt Entry Name
G6PT1_HUMAN
NCBI Summary for SLC37A4
This gene regulates glucose-6-phosphate transport from the cytoplasm to the lumen of the endoplasmic reticulum, in order to maintain glucose homeostasis. It also plays a role in ATP-mediated calcium sequestration in the lumen of the endoplasmic reticulum. Mutations in this gene have been associated with various forms of glycogen storage disease. Alternative splicing in this gene results in multiple transcript variants.[provided by RefSeq, Aug 2009]
UniProt Comments for SLC37A4
SLC37A4: Transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. Forms with glucose-6- phosphatase the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels. Defects in SLC37A4 are the cause of glycogen storage disease type 1B (GSD1B). GSD1B is a metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. GSD1 patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. GSD1B patients also present a tendency towards infections associated with neutropenia, relapsing aphthous gingivostomatitis, and inflammatory bowel disease. Defects in SLC37A4 are the cause of glycogen storage disease type 1C (GSD1C). Defects in SLC37A4 are the cause of glycogen storage disease type 1D (GSD1D). Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: transporter activity; glucose-6-phosphate transmembrane transporter activity
Biological Process: transport; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; glucose metabolic process; pathogenesis; glucose transport; glucose homeostasis; transmembrane transport
Disease: Glycogen Storage Disease Ic; Glycogen Storage Disease Ib
Research Articles on SLC37A4
1. Five SLC37A4 gene mutations were detected in 7 (25%) of the 28 children
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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