EIF1AX, Recombinant Protein

Eukaryotic translation initiation factor 1A X-linked; Eukaryotic translation initiation factor 1A X-linked; eIF-1A; eIF-4C; EIF1A; EIF1AP1; EIF4C

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl

1mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of EIF1AX recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

EIF1AX is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. It seems to be required for maximal rate of protein biosynthesis. And it enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits. Recombinant human EIF1AX protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Transcription & Translation

SDS-PAGE

18.6kDa (164aa), confirmed by MALDI-TOF

eukaryotic translation initiation factor 1A, X-linked

eukaryotic translation initiation factor 1A, X-chromosomal

Eukaryotic translation initiation factor 1A, X-chromosomal

EIF1A; EIF4C; eIF-1A X isoform; eIF-4C  [Similar Products]

This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.

Lahn BT., et al. (1997) Science 278 (5338):675-80.; Kim S., et al. (2007) Stem Cells Dev. 16 (4):537-45.;

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