Product Name
TUBB2B, siRNA
Full Product Name
TUBB2B siRNA (Human)
Product Synonym Names
Tubulin beta-2B chain
Product Gene Name
TUBB2B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BVA1
Specificity
TUBB2B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TUBB2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TUBB2B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TUBB2B sirna
siRNA to inhibit TUBB2B expression using RNA interference
Applications Tested/Suitable for TUBB2B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TUBB2B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_821080.1
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NCBI GenBank Nucleotide #
NM_178012.4
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UniProt Primary Accession #
Q9BVA1
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UniProt Secondary Accession #
A8K068[Other Products]
UniProt Related Accession #
Q9BVA1[Other Products]
Molecular Weight
49,953 Da
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin, beta 2B class IIb
NCBI Official Symbol
TUBB2B [Similar Products]
NCBI Official Synonym Symbols
PMGYSA; bA506K6.1
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NCBI Protein Information
tubulin beta-2B chain
UniProt Protein Name
Tubulin beta-2B chain
UniProt Gene Name
TUBB2B [Similar Products]
UniProt Entry Name
TBB2B_HUMAN
NCBI Summary for TUBB2B
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]
UniProt Comments for TUBB2B
TUBB2B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2B is implicated in neuronal migration. Defects in TUBB2B are a cause of polymicrogyria asymmetric (PMGA). PMGA is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination. Belongs to the tubulin family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: microtubule; cytoplasm; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; neuron migration; transmembrane transport; microtubule-based process
Disease: Polymicrogyria, Symmetric Or Asymmetric
Research Articles on TUBB2B
1. The association of polymicrogyria with thin or absent corpus callosum, dysmorphic basal ganglia, brainstem and vermis hypoplasia is highly likely to result from mutations in TUBB2B.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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