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产品名称: Mouse IFN Beta, E.Coli
产品型号: 12400-1
产品展商: PBL Assay Science
产品文档: 无相关文档
简单介绍
重组蛋白 Mouse Interferon Beta 由 PBL Assay Science(目录号 #12400-1)生产,仅供研究使用 (RUO),包装为 1e5 (100,000) 单位/瓶。Mu IFN-Beta 在大肠杆菌中表达。
Mouse IFN Beta, E.Coli
的详细介绍
产品介绍
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品牌
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PBL Assay Science
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货号
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12400-1
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品名
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Mouse IFN Beta, E.Coli
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规格
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1 x 105 units
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分子量
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19.6 kDa
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来源
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Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" (J. Interferon Res. 12: 139-43).
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纯度
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> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
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生物活性
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Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV.
EC50 for IFNβ is ~ 2.5U/ml
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缓冲液
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20 mM HEPES, pH 6.0; 0.5M NaCl; 6% glycerol; 0.1% bovine serum albumin (BSA)
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内毒素
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< 1 EU/μg
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纯度
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> 95%
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纯化方法
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A combination of ion exchange, hydrophobic interaction and size exclusion chromatography
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贮存
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For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles
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E.coli expressed Mouse IFN Beta protein with a single non-glycosylated polypeptide chain
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Increased stability with engineered disulfide bond
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95% or greater purity
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