Affinity purified mouse anti-calretinin (monoclonal)

The antibody 6B3pur was purified with Protein-G from in vitro produced monoclonal antibody 6B3 directed against calretinin (see product description 6B3 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.29 mg/ml in 0.1M phosphate buffered saline, pH 7.3. 

Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.029 g/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue. 

We recommend that the optimal dilutions be determined by titration experiments

After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.