Guinea pig antiserum against parvalbumin

 polyclonal

 human

 Guinea-pig

 Antiserum lyophil.

 This antibody was raised against recombinant mouse parvalbumin. GP72 reacts specifically with parvalbumin on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (Fig. 1). The antibody specifically localizes parvalbumin using free-floating or mounted sections of brain (Fig. 2), kidney and muscles of probably all vertebrates The antiserum does not stain the brain of parvalbumin-KO mice. Spectacular staining found in immunohistochemistry on floating sections of 4% PFAfixed specimens.

Immunohistochemistry: 1:3'000 - 1:10'000, when performed with the avidin-biotin method. 

Western blots: 1:1’000 to detect parvalbumin in brain extracts. We recommend that the optimal dilutions be determined by titration experiments.

 For long storage keep small aliquots of the reconstituted antiserum (e.g. 1 µl) at -80°C (or at least at -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freeze-thaw cycles. 

 1. *Filice F., Celio, M.R., Szabolcsi V. (2017) JCN, in press